Determination of biogenic amines in dry fish samples (Scomber scombrus)

The research focuses on the comprehensive analysis and quantification of biogenic amines in dry fish samples, an essential aspect food safety quality. To achieve accurate reliable results, a robust analytical method was adopted using high-performance liquid chromatography (HPLC) equipped with DAD UV/ Vis detector. extraction from samples optimized to ensure maximum recovery, suitable derivatization techniques were employed enhance detectability these compounds. Nine (9) amines; Histamine (HIS), Cadaverine (CAD), Tyramine (TYM), Ethylamine (ETH), Methylamine (MET), Putrescine (PUT), Typtamine (TYP), Agmatine (AGM) Isoamlamine (ISO) analyzed samples. correctness repeatability assessed by performing replicate analyses (n = 3) for two dried Mile 2 Choba sites respectively. detection levels ranged 1.mg/kg 1.70 mg/kg agmatine; 0.03 0.04 cadaverine; 5.52 5.60 Ethylamine; 0.42 0.48mg/kg histamine; 7.15 7.16 Methylamine; 5.13 5.29 Isoamalamine; 0.84 0.90 Putrescine; 2.70 2.90 tryptamine 4.26 4.44 tyramine across all sample locations histamine, significantly less than other bioamines detected. detected where tends be lowest high concentration recorded both though those regulation recommendation set EU regulations national standards products.